FastQCFastQC Report
Sun 20 Nov 2022
CO_007-COM.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCO_007-COM.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59622
Sequences flagged as poor quality0
Sequence length25-342
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC28844.837140652779175No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG28024.6996075274227636No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG25104.209855422495052No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG21073.5339304283653687No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC18913.171648049377746No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA17262.8949045654288685No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG17122.871423300124115No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG16662.79427057126564No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG11471.9237865217537151No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCG10901.828184227298648No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT10621.7812216966891417No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG10431.7493542652041192No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA10201.710777900774882No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAA9761.6369796383885142No Hit
CGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT9601.6101439066116534No Hit
TTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG9561.603434973667438No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT8681.4558384488947034No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT8531.4306799503538963No Hit
ATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG8051.3501727550233136No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAAT7571.2696655596927309No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT7171.2025762302505787No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG6771.1354869008084265No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGAC6731.1287779678642114No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC6471.085169903726812No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGC6341.063365871658113No Hit
CCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT6311.0583341719499513No Hit
CGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT6221.0432390728254672No Hit
GCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT6201.0398846063533596No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC6071.01808057428466No Hit
GTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT6031.0113716413404448No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATT6011.008017174868337No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG5910.9912448425077991No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGG5770.967763577203046No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAG5760.9660863439669921No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCG5350.897319781288786No Hit
AATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT5250.880547448928248No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT5070.8503572506792795No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCA4910.8235215189024185No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGA4710.7899768541813423No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT4680.784945154473181No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCGACC4260.7145013585589212No Hit
TCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT4250.7128241253228673No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT4190.7027607259065445No Hit
GAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT4090.6859883935460065No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT4080.6843111603099526No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTC4040.6776022273657375No Hit
GGTCGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT3950.6625071282412532No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG3860.647412029116769No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGCG3610.6054811982154238No Hit
CGCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT3500.587031632618832No Hit
CCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT3000.5031699708161417No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA2870.4813659387474422No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATG2800.4696253060950656No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT2800.4696253060950656No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCAT2750.4612391399147965No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG2730.4578846734426889No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGG2660.44614404079031234No Hit
CATGGCCGCCTCCCTAATGTTACACACTGACTAG2650.4444668075542585No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGG2640.44278957431820465No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGC2620.4394351078460971No Hit
GCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT2530.4243400087216128No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT2470.41427660930529No Hit
CCATGGCCGCCTCCCTAATGTTACACACTGACTAG2340.3924725772365905No Hit
TCCATGGCCGCCTCCCTAATGTTACACACTGACTAG2300.3857636442923753No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGA2280.3824091778202677No Hit
ATGGCCGCCTCCCTAATGTTACACACTGACTAG2270.3807319445842139No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCCA2120.3555734460434068No Hit
TGGCCGCCTCCCTAATGTTACACACTGACTAG2020.33880111368286875No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCC1930.3237060145583845No Hit
CTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1830.30693368219784645No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAG1580.2650028512965013No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1540.2582939183522861No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGC1540.2582939183522861No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGAGGCGGCCATGGAATTCGC1510.2532622186441247No Hit
GCGAATTCCATGGCCGCCTCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT1460.2448760524638556No Hit
GCCGCCTCCCTAATGTTACACACTGACTAG1380.23145818657542516No Hit
TCCCTAATGTTACACACTGACTAGAGACTAGTGGCAAT900.15095099124484251No Hit
GGCCGCCTCCCTAATGTTACACACTGACTAG860.14424205830062728No Hit
ATTGCCACTAGTCTCTAGTCAGTGTGTAACATTAGGGA760.12746972594008923No Hit
CTAGTCAGTGTGTAACATTAGGGAGGCGGCC670.11237462681560498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)